Please use this identifier to cite or link to this item: https://ir.sc.mahidol.ac.th/handle/123456789/853
Title: Effect of Carboxyl-Terminal Truncation on the Catalytic Performance of D - Phenylglycine Aminotransferase
Authors: Vithaya Meevootisom
Suthep Wiyakrutta
Keywords: D-phenylglycine aminotransferase;Carboxyl-terminus;Catalytic performance;Bioinformatics tools
Issue Date: 2016
Publisher: Faculty of Science and Technology, Suan Sunandha Rajabhat University
Citation: Suan Sunandha Science and Technology Jouranal. 2016;3(1):9-13.
Abstract: The D-phenylglycine aminotransferase (D-PhgAT) is a novel enzyme that can be used to synthesize precursors of antibiotics. This research addressed the function of the carboxyl-terminal (C-terminal) of DPhgAT. Its C-terminal amino acid sequence was compared to other related proteins using bioinformatics tools. The analyzed amino acid sequence was used to produce a genetically modified enzyme having a truncation of the 10 amino acid residues at the C-terminal region. The truncated D-PhgAT was purified and analyzed for catalytic performance. The results revealed that the truncated enzyme had better catalytic performance than the full-length enzyme by 37.49%. This research is a preliminary study for improving the enzymatic performance of D-PhgAT by structure-guided engineering and can be applied in the development of other enzymes.
URI: https://ir.sc.mahidol.ac.th/handle/123456789/853
ISSN: 2351-0889
Appears in Collections:Microbiology: National Journal Publications

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