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Title: Effects of pH and sucrose on seed germination of Paphiopedilum exul (Ridl.) Rolfe
Authors: Pahol Kosiyachinda
Keywords: seed propagation;asymbiosis;Lady's slipper orchid;Orchidaceae
Issue Date: 2017
Publisher: International Society for Horticultural Science (ISHS)
Citation: Kosiyachinda P, Imsomboon T, Thammasiri K. Effects of pH and sucrose on seed germination of Paphiopedilum exul (Ridl.) Rolfe. Acta Hortic.2017;(1167):95-100.
Abstract: Paphiopedilum exul (Ridl.) Rolfe is one of terrestrial or lithophytic orchids also known as the ''lady's slipper orchid''. The distribution is native around limestone mountain at western and eastern coasts of Thailand. The suitable conditions for P. exul seed germination were constructed. Seeds from 5-month-old fruits cultured on half-strength Murashige and Skoog agar medium (½ MS) at different pH levels (5, 5.6, 6.2, and 6.8) and supplemented with 10, 20, and 30 g L-1 sucrose. Seeds were cultured in dark conditions for 2 months and then cultured at 25±3°C under light intensity of 37 µmol m-1 s-1 for 16 h d-1 for 1 month. The results showed that different pH levels affected seed development to germination. The pH 5, 5.6, 6.2, and 6.8 gave seed germination of 28.07, 26.33, 21.75 and 19.12%, respectively. Sucrose concentrations did not affect seed germination significantly, but 20 g L-1 sucrose tended to give high seed germination at 24.97%. There is no interaction between sucrose and pH on seed germination.
ISSN: 0567-7572
Appears in Collections:Biology: International Proceedings

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