Please use this identifier to cite or link to this item: https://ir.sc.mahidol.ac.th/handle/123456789/776
Title: Micropropagation of Spathoglottis eburnea Gagnep, a Thai orchid species, through shoot tips
Authors: Kanchit Thammasiri Nathinee Panvisavas
Keywords: propagation;NAA;BAP;conservation;flow cytometry
Issue Date: Jul-2017
Publisher: International Society for Horticultural Science
Citation: พฤกษศาสตร์
Series/Report no.: Acta Horticulturae 1167;87-94
Abstract: Spathoglottis eburnea Gagnep is a Thai terrestrial orchid species grown in the north and northeast. It has a small population in the wild and can be endangered in the near future. To develop a protocol for Spathoglottis eburnea micropropagation, shoot tips were proliferated in half strength Murashige and Skoog (½MS) liquid media supplemented with the combination of α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) (0, 0.5, 1 or 2 mg L-1) and placed on a shaker at 110 rpm. After 3 months of culture, the highest shoot formation of 30.8 shoots explant-1 was obtained on ½MS medium supplemented with 1 mg L-1 NAA and 2 mg L-1 BAP. Whereas, the combination of 2 mg L-1 NAA and 0.5 mg L-1 BAP gave the highest root formation of about 11.1 roots explant-1. Moreover, the regenerated plants were analyzed by using flow cytometry and the results showed the same ploidy level.
Description: http://www.ishs.org/ishs-article/1167_13
URI: https://ir.sc.mahidol.ac.th/handle/123456789/776
ISSN: 0567-7572
Appears in Collections:Plant Science: International Proceedings

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