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|Title:||DNA extraction of plant species containing cardiac glycosides commonly found in Thailand|
|Keywords:||cardiac glycosides;DNA extraction;DNA amplification|
|Publisher:||The Botanical Society under the Royal Patronage of Her Majesty the Queen and The Botanical Garden Organization|
|Citation:||Thai Journal of Botany 2013;5(Special Issue):223-235.|
|Abstract:||To our interest, this study aimed to recover DNA from six plant species containing cardiac glycosides commonly found in Thailand, i.e. Calotropis gigantea (L.) R. Br. ex Ait., Cerbera odollam Gaertn., Nerium oleander L., Strophanthus gratus (Wall. & Hook.) Baill., Thevetia peruviana (Pers.) K. Schum., and Adenium obesum (Forssk.) Roem. & Schult. However, these plants have thick leaf blades, waxy surface, and exude either clear or milky sap when cut or tear, which can cause diffi culties in DNA extraction and affect to the yield and quality of DNA. Our results demonstrated that by conventional CTAB method of DNA extraction, DNA were obtained from young leaf tissue of Ca. gigantea, Ce. odollam, N. oleander and S. gratus, but not T. peruviana and A. obesum. DNA yields were 13.9±5.1, 50.0±35.2, 12.6±7.3, and 9.5±5.5 μg/g fresh tissue; the A260/A280 ratios were 1.8±0.04, 1.8±0.07, 1.7±0.25, and 1.8±0.04, respectively. These DNA extracts were PCR amplifi able. However, good quality DNA was successfully extracted from petals of the two problematic species, T. peruviana and A. obesum by two different methods, which were the conventional CTAB method and using the commercial DNA extraction kit, respectively. Total DNA yield were 47.8±12.1 and 2.7±0.6 μg/g fresh tissue; A260/A280 ratios were 1.9±0.05 and 1.8±0.05, respectively.|
|Appears in Collections:||Plant Science: National Journal Publications|
MU: National Journal Publications
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