Please use this identifier to cite or link to this item: https://ir.sc.mahidol.ac.th/handle/123456789/76
Title: Detecting DNA-​DNA hybridization at 3-​mercaptopropionic acid self-​assembled on tin-​doped indium oxide film with electrochemical measurement
Authors: Suthisa Leasen
Hector, José Hodak
Srisala Jiraporn
Toemsak Srikhirin
Kallaya Sritunyalucksana
Waret Veerasai
Somsak Dangtip
Keywords: 3-Mercaptopropionic acid;Tin-doped Indium oxide;Hybridization;Methylene blue;Differential pulse voltammetry
Issue Date: 2013
Publisher: Scientific
Citation: Suthisa Leasen et al., 2013, Advanced Materials Research, 770, 402
Abstract: Self-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events.
URI: http://ir.sc.mahidol.ac.th/handle/123456789/76
ISBN: 978-303785778-6
ISSN: 10226680
Appears in Collections:COE: International Proceedings
Physics: International Proceedings

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