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|Title:||Vasorelaxant activity and inhibitory effects on leukocyte function of a flavanoid compound from Kaempferia Parviflora|
|Keywords:||K.Parviflora;vasorelaxation;ICAM-1;neutrophil chemotaxis;superoxide anion generation;MPO;T-lymphocyte proliferation|
|Publisher:||The Thai Pharmacological and Therapeutic Society of Thailand.|
|Citation:||Wanikiat P, Jaipetch T, Reutrakul V. Vasorelaxant activity and inhibitory effects on leukocyte function of a flavanoid compound from Kaempferia Parviflora. Thai J Pharmacol 2010;32(1):321-326.|
|Abstract:||The rhizomes of Kaempferia parviflora (K. parviflora) (Zingiberaceae) have been used in Thai traditional medicine for gastrointestinal disorders, pain, allergy, and inflammation. The alcoholic infusion of its rhizome has also been used as a tonic for rectifying male impotence. Ethanolic extract of K. parviflora was reported to exhibit acute anti-inflammatory activities in the rat paw edema model (unpublished data) and vasorelaxant activity in isolated rat aortic rings. The purposes of this study were to investigate the effects of a flavanoid compound from K. Parviflora, (VR-F6/1) on vasorelaxant activity, the expression of ICAM-1 on human umbilical vein endothelial cells (HUVEC), CD62-L and Mac-1 on neutrophils, human neutrophil functional responsiveness and T-lymphocyte proliferation. VR-F6/1 was primarily investigated for its cytotoxic effects on HUVECs, neutrophils and T-lymphocytes using XTT cytotoxicity assay. The vasorelaxant activity of VR-F6/1 was assessed using myography technique. The effects of VR-F6/1 on fMLP-induced L-selectin shedding and Mac-1 expression on neutrophils were assessed using flow cytometry. Human neutrophil functional responsiveness was determined by measuring fMLPinduced chemotaxis, superoxide anion generation (SAG), and MPO release. PHA-induced-Tlymphocyte proliferation was quantified by [H3 ] thymidine incorporation. Viability of HUVECs, neutrophils, and T-lymphocytes were not significantly affected by VR- F6/1 (1- 100 μg/ml) (IC50>1000 μM). VR-F6/1 concentration-dependently caused acute relaxation of isolated mouse mesenteric arteries. VR-F6/1 (10 µM) inhibited ICAM-1 expression on HUVECs, and inhibited fMLP-induced L-selectin shedding and Mac-1 surface expression on neutrophils. VR-F6/1 concentration-dependently inhibited fMLP-induced chemotaxis, SAG, and MPO production in neutrophils. VR-F6/1 also significantly inhibited PHA-induced Tlymphocyte proliferation in a concentration-dependent manner. These findings suggest that anti-inflammatory activity of VR-F6/1 might be, in part, attributable to inhibition of ICAM-1 expression on HUVEC, inhibition of CD62L shedding and Mac-1 surface expression on neutrophils, neutrophil chemotaxis and SAG as well as T-lymphocyte proliferation. This study also demonstrates an acute vasorelaxant activity of VR-F6/1 where nitric oxide may mediate the effect.|
|Appears in Collections:||Chemistry: National Journal Publications|
Pharmacology: National Journal Publications
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