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|Title:||Molecular and biochemical characterization of type I cystatin (Stefin) of Fasciola gigantica|
|Keywords:||Fasciola gigantica;Cysteine protease inhibitor;Cystatin;Immunolocalization|
|Publisher:||The Thai Pharmacological and Therapeutic Society of Thailand.|
|Citation:||Thai J Pharmacol. 2010;32(1):269-271.|
|Abstract:||In the present study we describe type 1 cystatin, a cysteine protease inhibitor, as a major released antigen of the tropical liver fluke Fasciola gigantica (FgStefin-1). Immunohistochemical analysis showed that FgStefin-1 is abundant in tissue of tegumental type and the intestinal epithelium. Faint staining was observed in the epithelia of ovary and proximal uterus. Immunoblots showed the presence of FgStefin-1 in the parasite’s excretion/secretion (ES) product. Sera of experimentally infected sheep reacted with recombinant FgStefin-1 starting 8 weeks postinfection. Activity analyses of recombinant FgStefin-1 showed nanomolar inhibition constants for mammalian host and parasite cysteine proteases. Our results suggest protective functions of FgStefin-1, regulating intracellular cysteine protease activity, and possibly protection against extracellular proteolytic damage to the parasite’s intestinal and tegumental surface proteins.|
|Appears in Collections:||Biology: National Journal Publications|
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