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Title: Molecular and biochemical characterization of type I cystatin (Stefin) of Fasciola gigantica
Authors: Suksiri Vichasri-Grams
Keywords: Fasciola gigantica;Cysteine protease inhibitor;Cystatin;Immunolocalization
Issue Date: 2010
Publisher: The Thai Pharmacological and Therapeutic Society of Thailand.
Citation: Tarasuk M, Smooker P, Piedrafita D, Vichasri-Grams S, Viyanant V, Grams R. Molecular and biochemical characterization of type I cystatin (Stefin) of Fasciola gigantica. Thai J Pharmacol 2010;32(1):269-271.
Abstract: In the present study we describe type 1 cystatin, a cysteine protease inhibitor, as a major released antigen of the tropical liver fluke Fasciola gigantica (FgStefin-1). Immunohistochemical analysis showed that FgStefin-1 is abundant in tissue of tegumental type and the intestinal epithelium. Faint staining was observed in the epithelia of ovary and proximal uterus. Immunoblots showed the presence of FgStefin-1 in the parasite’s excretion/secretion (ES) product. Sera of experimentally infected sheep reacted with recombinant FgStefin-1 starting 8 weeks postinfection. Activity analyses of recombinant FgStefin-1 showed nanomolar inhibition constants for mammalian host and parasite cysteine proteases. Our results suggest protective functions of FgStefin-1, regulating intracellular cysteine protease activity, and possibly protection against extracellular proteolytic damage to the parasite’s intestinal and tegumental surface proteins.
ISSN: 0125-3832
Appears in Collections:Biology: National Journal Publications

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