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Title: Cloning and characterization of beta-1,3-glucanase, a known banana allergen from four banana cultivars in Thailand
Authors: Wisuwat Songnuan
Unchera Viboonjun
Keywords: Allergen;Allergy;Banana;Beta-1,3-glucanase;Epitope
Issue Date: 2015
Publisher: The Botanical Society under the Royal Patronage of Her Majesty the Queen and The Botanical Garden Organization
Citation: THAI JOURNAL OF BOTANY 2015;7(2):161-170.
Abstract: Banana (Musa L.) has recently been classifi ed as one of the foods causing allergic reactions. A number of patients with allergy to banana have been reported in Thai population, but the relevant allergens have not been described so far. To date, fi ve proteins have been characterized as banana allergens. Among them, beta-1,3-glucanase, is identifi ed as a potent cross-reactive allergen among rubber tree latex, pollens and some fruits. Thus, this study aimed to clone and characterize beta-1,3-glucanase cDNAs from four banana cultivars: ‘Kluai Hom Thong’, ‘Kluai Khai’, ‘Kluai Namwa’ and ‘Kluai Lep Mue Nang’. The cDNAs encoding beta-1,3-glucanase from four banana cultivars were successfully cloned. All cDNAs contained an open reading frame of 1,023 bp encoding a protein of 340 amino acids with the predicted molecular mass of 36.4 kDa and pI value of 8.83. Sequence analysis demonstrated that the deduced amino acids from four banana cultivars shared 97–99% identity, suggesting their potential for allergenic cross-reactivity. This is the fi rst report on molecular cloning of cDNA encoding an allergenic protein from banana cultivars in Thailand. However, the allergenicity of beta-1,3-glucanase among Thai patients suffering from banana allergy should be further investigated.
ISSN: 1906-7038
Appears in Collections:Plant Science: National Journal Publications

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