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|Title:||Cloning and characterization of β-cyanoalanine synthase gene in rubber tree (Hevea brasiliensis)|
|Keywords:||Beta-Cyanoalanine synthase;Hevea brasiliensis;Latex yield;Ethylene biosynthesis;Cyanide|
|Publisher:||Botanical Society of Thailand.|
|Citation:||Thai journal of botany. special, 5 (2013), 249-259|
|Abstract:||Overstimulation of rubber tree with ethylene can lead to the tapping panel dryness, a physiological disorder leading to the cessation of latex fl ow. Exogenous ethylene could induce endogenous ethylene biosynthesis causing the release of cyanide, a co-product of ethylene. In plants, cyanide could be detoxifi ed by beta-cyanoalanine synthase (CAS). In this study, full-length cDNA and promoter of HbCAS were cloned and characterized. Its deduced amino acid sequence shared 64.4-89.9% identity with CAS from other plants. The cloned promoter harbored several hormone-responsive elements including salicylic acid (SA) and auxin. Expression level of HbCAS was increased at 16 hours after SA and auxin treatments. The HbCAS expression might be up-regulated for detoxifi cation of cyanide released from endogenous ethylene biosynthesis induced by SA and auxin.|
|Appears in Collections:||Biotechnology: National Journal Publications|
Plant Science: National Journal Publications
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