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Title: Towards molecular sexing by MALDI-TOF
Authors: Nathinee Panvisavas
Keywords: MALDI-TOF;primer extension;amelogenin;forensic science
Issue Date: 2014
Publisher: การประชุมวิชาการบัณฑิตศึกษาระดับชาติและนานาชาติ ครั้งที่ 4
Citation: พฤกษศาสตร์
Series/Report no.: ;2622
Abstract: Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) is an alternative method for rapid and accurate nucleic acid analysis. Here, we demonstrated a potential of MALDI-TOF for robust gender identification of forensic DNA samples. The process is practical and straightforward. PCR product of amelogenin gene amplification was used as the template for primer extension reaction, and the extended product was analyzed with MALDI-TOF. Sex can be determined from the two distinguishable mass spectra patterns generated by male and female samples. This primer extension-coupled MALDI-TOF could analyze as low as 30 pg of genomic DNA with reproducibility
Appears in Collections:Plant Science: International Proceedings

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