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Title: Investigation into effect of sample storage time on DNA analysis from human femur
Authors: Nathinee Panvisavas
Keywords: Sample storage;DNA analysis;human femur
Issue Date: 2014
Publisher: การประชุมวิชาการบัณฑิตศึกษาระดับชาติและนานาชาติ ครั้งที่ 4
Citation: พฤกษศาสตร์
Abstract: Prolonged storage of bone samples after the first DNA analysis is inevitable in cases of unidentified human remains because these skeletal remains may be the only piece of evidence for positive identification. Often the re-testing for results confirmation would be required. However, the long term storage could impact the chance for obtaining useful results in future analysis due to the possibility of DNA degradation in the samples. Therefore, the present study aims at investigating the effect of sample storage time on human bone DNA analysis. For the purpose of comparison, post-storage DNA analysis was carried out on 10 femur samples of unidentified human skeletal remains, exactly as pre-storage analysis. The results indicated the occurrence of DNA degradation in both pre- and post-storage bone samples. The STR typing results of post storage analysis revealed increased nuclear DNA degradation as indicated by allelic and loci drop-outs, lower peak heights, peak imbalances, and degraded profile pattern. Poststorage mtDNA HV fragment analysis of 9 samples revealed that the HVI and HVII fragments were intact; in contrast, fragmentation of large HV fragment was evidence.These suggested the possibility of successful mitotyping for HV I and HV II regions.
Appears in Collections:Plant Science: International Proceedings

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