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|Title:||Isolation and molecular characterization of Clostridium difficile strains from patients and the hospital environment in Belarus|
|Keywords:||Clostridium difficile;toxin type;ribotype;multiplex PCR|
|Publisher:||Burapha University International Conference 2014|
|Abstract:||Clostridium difficile is a bacterial enteric pathogen that can cause nosocomial diarrhea. Currently, the prevalence and severity of C. difficile infection have increased rapidly due to the study were to determine the prevalence of C. difficile in hospitalized diarrhea patients admitted at Ramathibodi Hospital during January to December 2010 and to characterize these isolates for the presence of toxin genes and their molecular types. C. difficile was detected from 52 of 216 (24%) patient stool samples using multiplex PCR detection of toxin genes. Twenty four C. difficile isolates were recovered from C. difficile positive stool samples using selective culture method. Among the 24 C. difficile isolates, 9 were classified as tcdA+ tcdB+ cdtA/cdtB+, 8 as tcdA+ tcdB+ cdtA/cdtB, 1 as tcdA- tcdB+ cdtA/cdtB- and 6 as tcdA- tcdB- cdtA/cdtB-. Strain identity was determined by a PCR-ribotyping method. Fifteent different PCR-ribotype patterns were classified, among which ribotype 017 was the most common type that comprised 29% of C.difficile strains retrieved from in the hospital.|
|Appears in Collections:||Biology: International Proceedings|
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