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|Title:||Cloning and expression of a ABC transporter from clostridium difficile|
|Keywords:||ABC transporter;C.Difficile;Cloning;Drug resistance|
|Series/Report no.:||The 4th International Biochemistry and Molecular Biology Conference;|
|Abstract:||Clostridium difficile is the major infectious cause of nosocomial diarrhea, which is typically initiated by disruption of gastrointestinal flora via the use of broad-spectrum antibiotics. In recent year, it is increasingly difficult to treat C. difficile infection owing to its multidrug resistance. Active efflux includes ATP-binding cassette transporters which are a common mechanism of resistance to antibiotics in micro-organisms. The genome of C. difficile was screened for sequences encoding putative ABC transporter proteins homologous to multidrug resistance ABC transporter in C. hathewayi. The CD2593, ORFs homologous to efflux gene was cloned into the pET28A expression vector and expressed in Escherichia coli. In this study, the potential contribution of ABC transporters in antibiotic resistance in C. difficile was investigated. CD2593-expressing cells exhibited higher half minimal inhibitory concentration of antibiotics compared to the control cells. Therefore, CD2593 might function as a multidrug ABC transporter involved in multiple antibiotic resistance.|
|Appears in Collections:||Biology: International Proceedings|
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