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Title: Quantitative determination of lopinavir and ritonavir in syrup preparation by liquid chromatography
Authors: Duangjai Nacapricha
Prapin Wilairat
Keywords: Lopinavir;Ritonavir;Protease inhibitors;HPLC
Issue Date: 2009
Publisher: Faculty of Pharmacy, Mahidol University
Citation: Phechkrajang C.M., Thin E.E., Sratthaphut L, Nacapricha D, Wilairat P. Quantitative determination of lopinavir and ritonavir in syrup preparation by liquid chromatography. Mahidol University Journal of Pharmaceutical Science 2009;36(1-4):1-12.
Abstract: A simple high-performance liquid chromatographic method (HPLC) was developed for the simultaneous determination of lopinavir and ritonavir in syrup. A reversed-phase with isocratic elution was utilized. The mobile phase was a mixture of 10 mM ammonium acetate, pH 7 and acetonitrile (50:50, v/v). The flow rate was operated at 1 ml/min and the presence of two interest compounds was detected by a UV detector at 245 nm. The retention times on the described method were about 12.5 min and 14.7 min for ritonavir and lopinavir, respectively. The developed HPLC method was also validated for important performance characteristics such as linearity, accuracy and precision. Linearity of the developed method was evaluated by using the correlation coefficient greater than 0.999 for both drugs. Accuracy was expressed as the percent of recovery and closed to 100%. Good precision was also obtained with the% RSD of less than 2 for all cases. The developed method was then applied to determine lopinavir and ritonavir in syrup sample. The percent labeled amount of both drugs obtained from HPLC method were 100.5% and 100.3% for lopinavir and ritonavir, respectively.
ISSN: 0125-1570
Appears in Collections:Chemistry: National Journal Publications

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