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|Title:||Comparison of selective and nonselective enrichment broth media for the detection of campylobacter jejuni and C. coli|
|Abstract:||Campylobacter, gram-negative spiral rod bacteria, are now widely recognized as one of the leading causes of human bacterial gastroenteritis worldwide including Thailand. C. jejuni accounted for 90% of Campylobacter enteritis in humans and C. coli accounted for 2.5% of the isolates. These bacteria can be isolated from various food especially poultry meats. However, food normally contains small amount of C. jejuni and C. coli contamination, therefore, the enrichment step is usually required. Because the selective broth media for C. jejuni and C. coli is significantly more expensive than the nonselective media, it is interestingly to examine whether the use of the nonselective media could accomplish comparable detection of C. jejuni and C. coli to the selective media. Thus, the aim of this study was to compare the performance of the selective broth media (Bolton broth or BB) with the nonselective broth media (Brain Heart Infusion or BHI, Tryptic Soy Broth or TSB, and Nutrient Broth or NB) for the growth of C. jejuni and C. coli during the enrichment step. Pure cultures of C. jejuni and C. coli reference strains were used to establish the performance of the selective and nonselective broth media. Cell densities of C. jejuni and C. coli reference strains at concentrations of 106 CFU/ml for each species were added to each broth media. The ability of each broth to support the growth of C. jejuni and C. coli was estimated by determining cell densities in each culture media at 650 nm after incubation for 16 h. Then, the cell densities from each broth media were compared. In pure culture, BB supported significantly most growth of C. jejuni and C. coli while NB supported significantly less growth of C. jejuni and C. coli (P < 0.05). C. jejuni grew significantly better in BHI than in TSB (P < 0.05) while C. coli grew equally well in BB and BHI. The consistent results were obtained from PCR analysis after bacterial DNA extraction. Of 30 naturally-ontaminated fresh chicken samples examined by PCR analysis, C. jejuni was detected in 16 (53.3%) samples cultured in BB, 14 (46.7%) samples cultured in BHI and 5 (16.7%) samples cultured in TSB. No C. jejuni was detected in samples culture in NB. None of all samples was positive for C. coli.|
|Appears in Collections:||Microbiology: International Proceedings|
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