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|Title:||Beta-glucosidases from Thai plants|
|Publisher:||Faculty of Science, Srinakharinwirot University|
|Citation:||Srinakharinwirot Science Journal. 19-21, special (2009-2011)|
|Abstract:||Bata-Glucosidases (126.96.36.199) are classified as members of glycosyl hydrolase families 1 and 3, and are proposed to act via a retaining mechanism. Plant Beta-Glucosidases may have various natural substrates and fulfil many biological functions. These include an isoflavonoid Beta-Glucoside found in Thai rosewood (Dalbergia cochinchinensis Pierre), a cyanogenic glucoside in cassava (Manihotesculenta Crantz) and a steroid glucoside in Solanum torvum. Thai rosewood Beta-Glucosidase (dalcochinase) and cassava cyanogenic Beta-Glucosidase (linamarase) have been purified, and their properties studied. Dalcochinase catalyses reverse hydrolysis well, giving glucose disaccharides and trisaccharides from free glucose, while linamarase is the only enzyme studied thus far that can catalyse transglucasylation reactions using primary, secondary and tertiary alcohols as acceptors. Dalcochinase has been cloned, sequenced and expressed in yeast, and shows 60% homology to cassava and white clover linamarase. Thus, despite their structural similarities, the Beta-Glucosidases from different sources show interesting differences in their substrate specificity for the hydrolysis, reverse hydrolysis and transglucosylation reactions. So, they may serve as models for study of structure-function relationships.|
|Appears in Collections:||COE: National Journal Publications|
Biochemistry: National Journal Publications
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