Please use this identifier to cite or link to this item: https://ir.sc.mahidol.ac.th/handle/123456789/224
Title: Beta-glucosidases from Thai plants
Authors: Jisnuson Svasti
Keywords: Beta-glucosidase;Thai plants
Issue Date: 2011
Publisher: Faculty of Science, Srinakharinwirot University
Citation: Srinakharinwirot Science Journal. 19-21, special (2009-2011)
Abstract: Bata-Glucosidases (3.2.1.21) are classified as members of glycosyl hydrolase families 1 and 3, and are proposed to act via a retaining mechanism. Plant Beta-Glucosidases may have various natural substrates and fulfil many biological functions. These include an isoflavonoid Beta-Glucoside found in Thai rosewood (Dalbergia cochinchinensis Pierre), a cyanogenic glucoside in cassava (Manihotesculenta Crantz) and a steroid glucoside in Solanum torvum. Thai rosewood Beta-Glucosidase (dalcochinase) and cassava cyanogenic Beta-Glucosidase (linamarase) have been purified, and their properties studied. Dalcochinase catalyses reverse hydrolysis well, giving glucose disaccharides and trisaccharides from free glucose, while linamarase is the only enzyme studied thus far that can catalyse transglucasylation reactions using primary, secondary and tertiary alcohols as acceptors. Dalcochinase has been cloned, sequenced and expressed in yeast, and shows 60% homology to cassava and white clover linamarase. Thus, despite their structural similarities, the Beta-Glucosidases from different sources show interesting differences in their substrate specificity for the hydrolysis, reverse hydrolysis and transglucosylation reactions. So, they may serve as models for study of structure-function relationships.
URI: http://ir.sc.mahidol.ac.th/handle/123456789/224
ISSN: 0857-1600
Appears in Collections:COE: National Journal Publications
Biochemistry: National Journal Publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.